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Deep Learning Technology: Sebastian Arnold, Betty van Aken, Paul Grundmann, Felix A. Gers and Alexander Löser. Learning Contextualized Document Representations for Healthcare Answer Retrieval. The Web Conference 2020 (WWW'20)

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Comparative Evaluation of Diatherix Target Enriched Multiplex Polymerase Chain Reaction and BioFire FilmArray in the Detection of Viral and Bacterial Respiratory Pathogens

Background. The speed and efficiency of etiologic determination of influenza-like illness (ILI) has been greatly improved with multiplex diagnostic assays. We conducted a comparative evaluation of 2 multiplex assays with coverage of viral and bacterial respiratory pathogens.

Methods. Specimens (n = 403) were derived from an observational study of febrile ILI among otherwise healthy subjects at 5 US military hospitals from 2009 to 2014. Specimens were first tested by single-plex polymerase chain reaction (PCR) for influenza. Specimens were then tested by 2 assays: (1) target‐enriched multiplex PCR (TEM-PCR; Diatherix Laboratories, Inc, Huntsville, AL) and (2) BioFire FilmArray Respiratory Panel (FilmArray; BioFire Diagnostics, Salt Lake City, UT). The panels have 8 viral (human rhinovirus/enterovirus [HRV/EV], influenza A, influenza B, coronavirus, respiratory syncytial virus (RSV), parainfluenza, human metapneumovirus [hMPV], adenovirus [ADV]) and 3 bacterial (Mycoplasma pneumoniae, Bordetella pertussis, and Chlamydophila pneumoniae) pathogens in common. Specimens were selected based on availability, and tests were performed in separate laboratories. Kappa (κ) coefficients and 95% confidence intervals (CIs) were computed.

Results. The multiplex evaluation of influenza-PCR-positive specimens (n = 34) was incomplete and thus excluded from analysis. Of the 308 specimens with complete results, TEM-PCR and FilmArray detected at least 1 viral pathogen among 197 (64.0%) and 198 (64.3%) specimens, respectively. The frequency of viral codetection (i.e. 2 or more viral pathogens in a specimen) was 7.1% for TEM-PCR and 6.5% for BioFire FilmArray (p = 0.67; McNemar's test). Only 1 (0.3%) specimen was positive for C pneumoniae by FilmArray, and neither assay detected B pertussis. Pathogen-specific agreement between the panels was as follows: HRV/EV (κ = 0.75; 95% CI, 0.66–0.84), coronavirus (κ = 0.91; 95% CI, 0.86–0.97), RSV (κ = 0.85; 95% CI, 0.77–0.94), parainfluenza (κ = 0.97; 95% CI, 0.91–1), hMPV (κ = 0.85; 95% CI, 0.74–0.96), ADV (κ = 0.21; 95% CI, −0.15 to 0.58), and M pneumoniae (κ = 0.72; 95% CI, 0.42–1).

Conclusion. These results reveal a high degree of concordance between Diatherix Laboratories TEM-PCR and BioFire FilmArray in the detection of viral respiratory pathogens.

Disclosures. L. Malone, Diatherix Laboratories, LLC: Employee, Salary; E. Grigorenko, Diatherix Laboratories, LLC: Employee, Salary; D. Stalons, Diatherix Laboratories, LLC: Employee, Salary