Dataset: 11.1K articles from the COVID-19 Open Research Dataset (PMC Open Access subset)
All articles are made available under a Creative Commons or similar license. Specific licensing information for individual articles can be found in the PMC source and CORD-19 metadata
More datasets: Wikipedia | CORD-19

Logo Beuth University of Applied Sciences Berlin

Made by DATEXIS (Data Science and Text-based Information Systems) at Beuth University of Applied Sciences Berlin

Deep Learning Technology: Sebastian Arnold, Betty van Aken, Paul Grundmann, Felix A. Gers and Alexander Löser. Learning Contextualized Document Representations for Healthcare Answer Retrieval. The Web Conference 2020 (WWW'20)

Funded by The Federal Ministry for Economic Affairs and Energy; Grant: 01MD19013D, Smart-MD Project, Digital Technologies

Imprint / Contact

Highlight for Query ‹Deltavirus infectious disease symptoms

Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections

Patient Enrollment and Specimen Collection

For 1 year (January 2016 to January 2017), we prospectively enrolled children age 6 months to 18 years who were admitted to the Monroe Carell Jr. Children’s Hospital at Vanderbilt (MCJCHV) with acute MSKI undergoing a surgical drainage/biopsy procedure. Potential study subjects were identified by daily communication with the pediatric orthopedic surgery service.

Source specimens were collected per standard of care in accordance with the preference of the treating physician (bone biopsy for osteomyelitis, fluid aspiration for septic arthritis, subperiosteal abscess [when applicable], and pyomyositis) in the operating room and sent to the Vanderbilt University Medical Center Clinical Laboratory for bacterial culture. Additionally, 2 mL of synovial fluid (in cases of septic arthritis) or a swab of the infected area of bone (in cases of osteomyelitis) was placed into an ESwab transport tube (Copan, Brescia, Italy) for TEM-PCR and stored at –80°C until processing. Following enrollment, subjects were monitored using the Vanderbilt Electronic Medical Record to determine whether a pathogen was isolated by culture.

Informed consent was obtained from the parents, and informed assent was obtained from the participant when possible, before inclusion in the study. The study was approved by the Vanderbilt University Medical Center Institutional Review Board (IRB).

Specimen Processing for TEM-PCR

Blinded clinical samples were submitted to Diatherix-Eurofins for TEM-PCR detection of Kingella kingae, Haemophilus influenzae, Streptococcus pyogenes, Streptococcus pneumoniae, S. aureus, methicillin (mecA) and clindamycin/erythromycin (ermA, ermC) resistance genes, and the Panton-Valentine leukocidin (PVL) locus. Briefly, nucleic acid was extracted from 275 µL of clinical sample, and 20-µL PCR reactions were prepared using 2X Platinum Multiplex Master Mix (ThermoFisher Scientific, Waltham, MA), 2.4 µL of TEM-PCR primer mix, and 4 µL of template DNA. Amplification was performed in 96-well plates on the GeneAmp PCR System 9700 thermocycler (ThermoFisher Scientific, Waltham, MA). In a single reaction, each target was amplified with nested primers and subsequently amplified with a pair of universal primers containing proprietary sequences to amplify all targets in the single reaction. The reverse universal primer was biotinylated for downstream detection. Biotinylated amplicons were detected by hybridization to a detection probe covalently coupled to a glass microarray substrate, and streptavidin-phycoerythrin conjugate was added. Fluorescent signal emission was measured on a SensoSpot imaging reader (Sensovation AG, Radolfzell, Germany).

Data Analysis

Demographic data were collected from the electronic medical record system at MCJCHV. Sensitivity and negative predictive values were calculated to compare TEM-PCR to culture.

Patient Characteristics

During the study period, 25 source specimens were collected for culture and TEM-PCR on 24 patients (1 subject was admitted twice, 2 months apart). Fourteen (58%) participants were male, with a median age (interquartile range) of 8 (3–12) years. Seventy-one percent of the patients were non-Hispanic white, 25% were non-Hispanic black, and 4% were multiracial. Fifty-two percent of the samples came from patients with septic arthritis (8 hips, 4 knees, 1 shoulder); 28% had osteomyelitis (3 femoral, 2 tibial, 1 fibular, 1 foot); 16% had combined osteomyelitis and septic arthritis (2 upper extremity, 2 lower extremity), and 4% had an isolated pyomyositis (gluteal). Sixty percent of patients were treated with at least 1 dose of antibiotics before source sample collection.

Pathogen Identification and Antibiotic Susceptibility Testing by Culture

An organism was isolated by bacterial culture in 17 of 25 specimens (68%) (Table 1); all were S. aureus. Five of the 17 S. aureus isolates were methicillin-resistant (MRSA), 2 were clindamycin-resistant (both MSSA), and 8 were erythromycin-resistant.

Pathogen Identification and Antibiotic Resistance Detection by TEM-PCR

Using TEM-PCR, an organism was detected in 20 of 25 specimens (80%) (Table 1). S. aureus was detected in 18 specimens, and K. kingae was detected in 2 specimens. Of the 18 S. aureus positive specimens, 5 were methicillin-resistant (MRSA), 2 were clindamycin-resistant, and 2 were erythromycin-resistant. Nine of 18 (50%) S. aureus isolates were positive for the PVL gene.

Performance of TEM-PCR Compared With Culture

TEM-PCR was concordant with 17 of the 17 (100%) pathogens isolated by culture. Three pathogens were detected by TEM-PCR that were not detected by culture, all of which received antibiotic therapy before source specimen collection. Of these 3, 2 were K. kingae and 1 was S. aureus, which was previously isolated in the same patient by culture and TEM-PCR in their previous hospital admission. Five specimens were negative by both culture and TEM-PCR. There were no specimens with a positive culture and negative TEM-PCR.

Overall, TEM-PCR exhibited 100% concordance with positive culture samples and identified 3 pathogens that, while negative by culture, were considered by study investigators to be highly likely to be the causative pathogen based on clinical characteristics. Compared with culture, TEM-PCR had a sensitivity and negative predictive value of 100%. Specificity and positive predictive values were not calculated, as the specimens positive by TEM-PCR and negative by culture were not considered “false positives.”

Compared with culture, TEM-PCR identified 5 of 5 (100%) methicillin-resistant S. aureus, 2 of 2 (100%) clindamycin-resistant S. aureus, and 2 of 8 (25%) erythromycin-resistant S. aureus (Table 1).


This study provides insight into the utility of TEM-PCR for children with MSKI. The results show that TEM-PCR was highly concordant with culture-based testing (reference standard) in pathogen identification for children with MSKI and outperformed traditional culture, increasing overall pathogen detection from 68% to 80%. Additionally, TEM-PCR reliably detected methicillin and clindamycin resistance in S. aureus isolates. These results suggest that TEM-PCR has the potential to inform antibiotic selection early in the disease course, decreasing the use of broad-spectrum empiric antibiotic regimens and promoting antimicrobial stewardship.

The identification of a causative organism in MSKI is critical and allows for optimal targeted antimicrobial therapy. Traditionally, clinicians have relied on bacterial culture for pathogen isolation and antibiotic susceptibility testing, although blood and operative cultures frequently remain negative [1–3]. Several reasons for this exist, including antibiotic administration before tissue sampling and the fastidious nature of causative pathogens.

K. kingae is a fastidious gram-negative organism that is increasingly recognized as an important pathogen causing MSKI, especially in children <4 years of age. Despite the importance of this pathogen, the culture yield remains very low, even under optimal conditions. PCR has been shown to greatly increase the rate of pathogen identification in cases of MSKI due to K. kingae. Our study supports this finding, as K. kingae was identified by TEM-PCR in 2 cases where cultures remained negative.

In addition to increasing pathogen identification, TEM-PCR can rapidly provide information on antibiotic susceptibility testing (AST), allowing for timely optimization of antibiotics. Clinical specimens submitted for TEM-PCR testing are batched by panel type and processed daily, and test results are reported to physicians within 7 hours of specimen receipt in the laboratory. Culture-based methods often take 24–48 hours to identify a pathogen and an additional 24–48 hours until AST is complete. This 48–96-hour delay leads to prolonged broad-spectrum empiric antimicrobial exposure until AST is secured. In our study, TEM-PCR was 100% concordant with culture-based methods for methicillin and clindamycin resistance testing. The lower erythromycin resistance detection by TEM-PCR is most likely due to the limited targets selected for this assay (ermA and ermC), though this may also be a result of inadequate sensitivity of this component of the multiplex assay. The additions of ermB, msrA, msrB, and mphC gene targets would likely increase erythromycin resistance detection; however, given that erythromycin is not used in the treatment of MSKI in children, inclusion of these targets has limited clinical utility. Overall, TEM-PCR allows for rapid AST results, decreasing time to optimal therapy and increasing antimicrobial stewardship.

There are several limitations to this proof-of-concept study. The limited sample size resulted in the identification of only 2 pathogen species—S. aureus and K. kingae. Although TEM-PCR has been shown to reliably detect numerous bacterial and viral species in other infectious diseases [11–13], the sensitivity in pediatric MSKI remains unknown. Additionally, despite the strong concordance with culture-based methods, the small sample size limits the power of the study. Despite these limitations, the high sensitivity and negative predictive values seen in this small pilot study suggest that TEM-PCR, as a rapid diagnostic tool, can play an important role in the initial evaluation of children with MSKI.

Given the challenges of pathogen detection by conventional culture in children with MSKI and the increasing number of antibiotic-resistant organisms, there is a major need for optimizing diagnostic strategies. Recent studies have investigated the utility of K. kingae–specific PCR in children with MSKI, as well as broad-range PCR for prosthetic joint infections [8, 14]. Few studies, however, have described the utility of a flexible, well-developed platform in detecting the common pathogens of pediatric MSKI. TEM-PCR provides rapid results with high sensitivity utilizing a multiplex panel that targets likely pathogens and known antibiotic resistance mechanisms, and further investigation of this technology is warranted.